1. Define tissue culture, primary culture, subculture, cellline, confluence and cell passaging
2. What are the components of HeLa cell culture media andexplain their function.
3. Basic Tissue culture Lab Equipment: info about all theequipment that we need in the cell culture room.
4. Sterilization: What is aseptic technique? What iscontamination? Write all the sterilization techniques that we applyfor the sterilization of our equipment.
5. Give a step wise procedure for retrieving cells from frozenstocks, cell passaging and freezing the cells
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