QUSTION7:

A)What is common to both EI and ESI? Please choose one: a. Theyare both combined with quadrupole analyzers b. They are bothcombined to HPLC c. They are both applied at atmospheric pressureD. They are both soft ionization techniques e. Gas phase formationand ionization occur simultaneously in both f. They are both hardionization techniques g. They are both applied under vacuum h. Theyare both combined to GC

B)Which one of the following is the application of fluorescencespectroscopy? I. High concentrations of protein-metal complexes indrugs II. Determination of fluorescent impurities III. Fluorescentdrugs in low dose formulations Please choose one: a. I and III b. Iand II c. Only II D. I, II and III e. II and III

C)Mass spectrometry is combined with chromatography to separatethe molecules in complex mixtures and then structurallycharacterize them by NMR. Why hyphenated techniques of NMR withliquid chromatography is not widespread?

Lütfen birini seçin: a. Pumping system of HPLC damages NMR b.Magnetic field used in NMR causes fluctuations in NMR c. The ionsource requires conversion of the sample to the gas phase d.Interfaces between NMR and chromatography destroys samples e.D-labelled solvents used is expensive f. NMR is a not a continuousinjection technique

D)What can not be said about the determination of ethinylestradiol in tablets using fluorescence spectroscopy? Pleasechoose one: a. Emission peak at 310 nm is due to fluorescence, whenexcited with 285 nm b. Interferences in ethinyl estradiol intablets absorb at UV Vis region c. Fluorescence emission spectrumdemonstrates two peaks, when excited with 285 nm D. Emission peakat 285 nm is due to scattering, when excited with 285 nm e.Ethinylestradiol does not demonstrates fluorescence emission priorto derivatization with strong fluorescent compound quinine

E) In determination of stability of recombinant fibroblast byfluorometry, which one of the following is correct? I. Emission andfluorescence peaks of tyrosine Residues Residues are MEASURED II.Protein denaturation causes changes in the amount of tyrosine andtryptophan III. Emission energies of tryptophan is higher than itsexcitation energy Please choose one: a. I and II b. I, II and IIIc. I and III D. Only I e. II and III