1. Define tissue culture, primary culture, subculture, cellline, confluence and cell passaging

2. What are the components of HeLa cell culture media andexplain their function.

3. Basic Tissue culture Lab Equipment: info about all theequipment that we need in the cell culture room.

4. Sterilization: What is aseptic technique? What iscontamination? Write all the sterilization techniques that we applyfor the sterilization of our equipment.

5. Give a step wise procedure for retrieving cells from frozenstocks, cell passaging and freezing the cells