UV/Vis spectrophotometry is used frequently for the quantitativeanalysis of biochemically important molecules, such as reducednicotinamide-adenine dinucleotide (NADH) and cystidinemonophosphate (CMP) A 56.0 mM solution of NADH has an absorbance of0.285 at a wavelength of 340 nm and 0.683 at 260 nm while anequimolar solution of CMP has an absorbance of 0.647 at 260 nm andan absorbance at 340 nm that is indistinguishable from the reagentblank. A sample that might contain NADH only, CMP only, or amixture of both NADH and CMP, gives an absorbance of 0.371 at 340nm and 1.50 at 260 nm. Which species are present and at whatconcentrations?
没有找到相关结果